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Applications of FISH in Tumor Diagnostics
- Feb 11, 2019 -

it goes without saying that in neoplasia the identification of cytogenetic markers is of high clinical significance for diagnostics, follow-up studies and prognosis., molecular cytogenetics is more and more performed independently from banding cytogenetic analyses in all kinds of tumors, too. . FISH approaches are especially suited to characterize chromosomal and subchromosomal copy number changes and gene fusions due to translocations or other rearrangements. All these features are characteristically found acquired aberrations in cancer . Centromeric region-directed probes in tumor diagnostics are mainly applied for determination and/or confirmation of clones with mono-, tri-, or tetrasomies of single chromosomes; also they are suited for follow-up of sex-mismatched bone marrow transplantation; to detect and characterize tumor-specific translocations whole chromosome paints . Another feature of FISH and cytogenetics is the analysis on single cell level, which is, in case of mosaic aberrations, as to be expected in tumor diagnostics, very useful to detect even small cell clones; if tissue sections are studied, FISH gives additional information on tumor morphology/heterogeneity.

Reference : Fluorescence In Situ Hybridization (FISH)  by Thomas Liehr Editor