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Molecular probe
- Aug 15, 2018 -

Molecular probes are functional substances that accurately answer biomedical questions. Molecular probes are a prerequisite and core technology for molecular imaging. In addition to the need for advanced imaging equipment, the development of molecular imaging technology requires the development of new and efficient molecular probes. There are many kinds of molecular probes. According to different imaging devices, molecular probes are classified into different types such as optics, nuclear medicine, magnetism, acoustics, and photoacoustics.


Introduction

Definition of scientific and technical terms Chinese name: molecular probe English name: molecular probe definition: a molecule that can be combined with other molecules or cell structures for the analysis of the location, properties, etc. of these molecules or cell structures. Usually labeled (eg radioactivity, fluorescence, antigen, enzyme labeling, etc.) for tracking detection. Oligonucleotides, labeled antibodies, and the like used for nucleic acid hybridization are commonly used molecular probes. Applied disciplines: biochemistry and molecular biology (a subject); methods and techniques (two subjects) The above content was approved by the National Science and Technology Terminology Committee


usefulness

Molecular probes are based on molecular hybridization techniques using probes to detect nucleic acid sequences having complementary sequences. The probe can be either a cloned or PCR amplified DNA molecule, or a synthetic oligonucleotide or an in vitro transcribed RNA molecule. The probe must be pure and free of other different nucleic acids. In order to ensure that the gene of interest is detected by base complementation, the probe must be a single-stranded molecule. Therefore, the double-stranded DNA probe must be single-stranded before application, and the double-stranded DNA probe is generally denatured by heating. The RNA molecules of the original single-stranded oligonucleotide and RNA probe need not be denatured. be usable. By labeling the probe with a radioisotope, the hybrid molecule can be observed by autoradiography. In modern times, non-radioactive isotope labeling systems have been developed. The commonly used probe labeling system uses a digoxigenin (DIG) labeled probe.