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Application Of Chromosomal Centromere Probe In The Diagnosis Of Bladder Tumor
- Aug 15, 2018 -

To explore the feasibility and effectiveness of Fluorescence in situ Hybridization (FISH) in the diagnosis of bladder tumors. METHODS: Randomized primers were used to label the centromere probes on chromosomes 3 and 7. The fluorescence in situ hybridization was performed on 66 cases of suspected bladder tumor patients with urinary exfoliated nuclei, and the cystoscopy biopsy or postoperative surgical examination was performed to confirm whether they had a diagnosis. Bladder tumors and their staging, grading, and urinary exfoliative cytology. Conclusion: The sensitivity of FISH technique for the diagnosis of bladder tumors was 72.4%, and the specificity was 62.5%. Among the 58 patients with confirmed bladder tumors, the mutation rates of chromosomes 3 and 7 were 67.24% (39/). 58), 68.96% (40/58), the degree of distortion was positively correlated with pathological grade of bladder tumor (P<0.05), but there was no significant correlation with stage (P>0.05). The sensitivity of urine exfoliative cytology was 13.79% (8/58) and specificity was 100%. Conclusion: The diagnosis of bladder tumor by FISH is a non-invasive and effective diagnostic technique. It has higher sensitivity than urine exfoliative cytology, but there is no significant difference in specificity.