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What Is The Difference Between A Fluorescence In Situ Hybridization Probe And A Fluorescent Probe
- Aug 15, 2018 -

What is the difference between a fluorescence in situ hybridization probe and a fluorescent probe?


Fluorescence in situ hybridization was introduced in the late 1970s. It has been used in the study of chromosomal abnormalities. In recent years, with the increasing number of probes and clocks used in FISH, especially the full Cosmid probe and chromosome in situ suppression hybridization technology. The emergence of FISH technology not only in cytogenetics, but also widely used in oncology research, such as gene diagnosis gene mapping. The original radioisotope in situ hybridization technique has many disadvantages, such as re-labeling the probe for each test. The labeled probe shows obvious instability, requires more time and exposure time and environment. Pollution and so on.


When observing the results, more divisions are needed for statistical analysis. In addition, due to the different planes of radioactive silver particles and chromosome aggregation, errors in counting and the like may be caused. Compared to FISH, it has:

1 easy to operate, stable after labeling, can be used for two years after one mark;

2 method sensitive, can get results quickly;

3 On the same specimen, several different probes can be detected simultaneously;

4 can be used not only for the study of chromosome number or structure changes in dividing cells, but also for the study of chromosome number and genetic alteration of interphase cells.